Cyto-Plex
and Cyto-Cal Microspheres For Flow Cytometry and
Haematology
Cyto-Plex:
4 µm Carboxylated Microspheres for Suspension
Array Analysis
Suspension
array analysis is a powerful technique enabling
the simultaneous detection and quantitation of
multiple analytes with a single sample. Using
color coded microspheres with the appropriate
dyes, these tests can now be carried out using
commonly available flow cytometers.
Cyto-Plex Carboxylated Microspheres from Duke
Scientific provide twelve levels of red fluorescence
intensity for analysis of twelve different analytes.
Available singly or in kits, these highly uniform
particles have fluorescence intensities which
are completely separated from each other. The
use of a single diameter 4 µm microsphere
for all dye levels saves time by only requiring
the development and optimization of one bead chemistry.
High-density binding sites and low non-specific
binding enable coupling to a wide variety of antibodies,
nucleic acids and other biomolecules.
To download a brochure in Adobe Acrobat format
giving full details of Cyto-Plex 4µm Carboxylated
Microspjheres, please click here
.
Cyto-Plex: 5 µm Carboxylated Microspheres
for Suspension Array Analysis
Cyto-Plex
carboxylated microspheres are now also available
as highly uniform 5 µm
diameter beads.
To download a brochure in Adobe Acrobat format
giving full details of Cyto-Plex 5µm Carboxylated
Microspjheres, please click here
.
Cyto-Cal
Multifluor Intensity Calibrator
The
Cyto-Cal™ Multifluor Fluorescence Intensity
Calibrator is designed to simultaneously monitor
flow cytometer stability and provide a check on
instrument sensitivity and performance. Although
the product can provide relative fluorescence
estimations of labeled cells (MEFL)—it is
not designed for quantitation of fluorochromes
on cells.
The calibrator is a mixture of uniform 3 µm
microspheres with three dyes in seven different
fluorescent intensities. Due to the high uniformity
of the microspheres, singlet gating is not required.
The Calibrator consists of a single vial of fluorescent
beads precisely stained with fluorescent dyes
that have optimized intensity and broad emission
in multiple channels (FITC, PE, PE-Cy5, PE-Texas
Red, APC, APCCy7).
Each intensity level has an MEFL (mean equivalent
fluorochrome)* value for estimation of relative
fluorescence intensities of labeled cells.
To
download a brochure
giving more information on the Cyto-Cal Multifluor
Intensity Calibrator in Adobe Acrobat (.PDF) format,
click
here
Cyto-Cal Low Intesity Calibrator
In
many flow cytometry applications, it is critical
to be able to separate dimly labeled cells from
unlabeled (autofluorescent) cells. Cyto-Cal Low Intensity
Calibrator enables simultaneous monitoring of
FL1 (FITC), FL2 (PE), FL3 (PECy5) and FL4 (APC)
channels for adequate fluorescence sensitivity
and discrimination.
The
Calibrator contains an equal mixture of 6 µm
undyed microspheres and microspheres dimly labeled
with green, orange and red fluorescent dyes which
are designed to imitate a mixture of unlabeled
and dim labeled cells. The dyes are incorporated
within the polymer matrix (hard-dyed) for long-term
stability, using Duke Scientific’s proprietary
Firefli™ process.
To
download a brochure
on the Cyto-Cal Low Intensity Calibrator, please
click
here.
Cyto-Cal
Alignment Beads
Duke
Scientific alignment beads provide a superior
method for optical alignment and flow cell focusing
of flow cytometers. These 3µm
microspheres are of the highest quality in size
and fluorescence uniformity to permit the best
possible optimization of each parameter being
measured.
The Cyto-Cal 488 Alignment beads are excited by
the 488 spectral line of the argon laser and have
broad emission, allowing them to be used to align
the FL1 (FITC), FL2 (PE) and FL3 (PE-Cy5) channels
simultaneously.
The Cyto-Cal 633 Alignment beads are optimally
excited with the 633 nm He-Ne laser (635 nm diode
laser) and have maximum emission at 700 nm. They
are designed to align the FL4 (APC) channel. Cyto-Cal
Alignment beads are dyed internally with chemically
stable dyes and therefore have excellent signal
stability.
To
download a brochure in PDF format
on Cyto-Cal Alignment Beads, please click
here.
Cyto-Cal
Count Control
The
Cyto-Cal™ Count Control is designed for
absolute cell counting on flow cytometers. This
product contains uniform 7 µm microspheres
containing two encapsulated dyes. The single vial
contains fluorescent beads which are precisely
stained with fluorescent dyes that have optimized
intensity and broad emission in multiple channels
(FITC, PE,
PE-Cy5).
To
download a brochure in PDF format
on Cyto-Cal Count Control, please click
here.
Updated
12.02.07
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